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5950 sw 20th ave #77 • gainesville, fl 32607
phone (352) 281-0366 • e-mail nash@ufl.edu
kevin nash
profile
highly skilled in a broad range of preparative and analytical
techniques in protein chemistry and also molecular biology, having studied
phosphatases, proteases (hcv and hiv) and dna polymerases. able to work on
own initiative and as part of a team. proven leadership skills. managing,
teaching and motivating lab technicians, undergraduate and graduate students.
involved in the management of the 2nd international conference on protease
inhibitors (held at the university of florida by prof b. m. dunn).
first-class analytical, design and problem solving skills. good communication
skills. dedicated to maintaining high quality standards. very good computer
skills.
current
employment
·
postdoctoral associate in prof. nicholas muzyczka's
laboratory. powell centre for gene therapy. university of florida
·
i am currently working on the identification of host cell
factors that are involved in the replication of adeno-associated virus (aav).
aav is an important vector for genetic delivery in gene therapy. i am also
working on designing ribozymes for gene therapy of alzheimer’s disease.
previous
employment
·
post-doctoral associate university of florida, biochemistry
dept. 1997-1999.
·
tutoring assistant, biochemistry dept. university of queensland
1992-1996.
·
studentship at csiro (longpocket, qld.) 1991-1992.
education
·
university of florida, post-doc (2000-) molecular genetics and
microbiology, prof nicholas muzyczka.
·
university of florida, post-doc (1997-1999) biochemistry
dept., prof. ben m dunn.
·
university of queensland, ph.d. (1993-1996) biochemistry dept.,
assoc. prof susan hamilton. title: "structure function studies on
purple acid phosphatase"
·
university of queensland. honors (first class) (1992)
biochemistry title: "development of non-radioactive assays for
protein phosphotyrosine phosphatases"
·
university of queensland. b.sc. (1989-1991) majoring in
biochemistry and microbiology
major
scientific techniques
·
enzyme purification and kinetic analysis (phosphatases,
proteases and polymerases).
·
cloning and culturing techniques for protein expression in
bacteria, yeast and the baculovirus insect cell expression system
·
tissue culture (mammalian and insect cells)
·
recombinant aav and adenovirus
·
ribozymes
·
analytical and preparative hplc and fplc
·
elisa and western blots
awards
received
·
australian postgraduate research award scholarship
awarded to carry out doctoral studies at the university of queensland,
1993-1996.
·
postgraduate student poster award from the australian
society for biochemistry and molecular biology conference in 1993.
·
wool research and development national summer
undergraduate studentship award 1991-92.
previous
nonscientific employment
·
clifton park turf supplies, casual laborer (1986-1992).
interests
and activities
my strongest passions are travel,
photography, yoga and scuba diving. recently i have started learning sign
language as a second language. i hope it will give me an opportunity to
interact with the deaf community. i am also fond of working with computers,
and have particularly enjoyed web page design projects.
publications
papers-
·
k. nash, w. chen, w. m. macdonald and n. muzyczka (2001)
host cell enzymes involved in adeno-associated virus dna replication.
manuscript in preparation.
·
b. m. dunn, m. w. pennington, d. c. frase and k. nash
(1999).
comparison of inhibitor binding to fiv and hiv proteases: structure-based
drug design and the resistance problem. biopolymers, 51(1): 69-77
·
k. marshall, k. nash, g. hausmann, i. cassady, d. hume,
j. de jersey and s. hamilton (1997).
recombinant human and mouse purple acid phosphatases: expression and
characterization. archives of biochemistry & biophysics, 345(2):
230-236.
·
k. nash, m. feldmuller, j. de jersey, p. alewood and s.
hamilton (1993)
continuous and discontinuous assays for phosphotyrosyl protein
phosphatases using phosphotyrosyl peptide substrates. analytical biochemistry,
213: 303-309.
posters-
·
kevin nash, bin xu and ben dunn (1999) product inhibition of
the hepatitis c ns3 protease. 2nd international conference on
protease inhibitors.
·
d. constanza frase, kevin nash,
alfred chung and ben dunn (1999) inhibition of hiv and fiv proteases by
statine-based and methylene-amino based compounds. 2nd
international conference on protease inhibitors.
·
d. constanza frase, kevin nash,
alfred chung and ben dunn (1999) characterization of fiv protease mutants. 8th
international aspartic protease conference.
·
j. clemente, l. wikkeling, p. o’brien, a. herman, d.c.
frase, z. zimmerman, k. nash, m. goodenow, b.m. dunn (1999) kinetic
analysis of hiv protease variants isolated from a pediatric patient
undergoing ritonavir treatment. 8th international aspartic
protease conference.
·
z. zimmerman, k. nash, b. grieninger, s. rose, m.
goodenow, b.m. dunn (1999) gag-pol processing of hiv protease variants
generated from drug therapy. 8th international aspartic
protease conference.
·
kevin nash, bin xu, li zhang and ben dunn (1999) expression
of hepatitis c ns3 protease constructs. 13th annual protein
society meeting.
·
k. nash, m. feldmuller, m. bowen, k. marshall, j. de
jersey and s. hamilton (1995) expression of purple acid phosphatase: an
enzyme involved in bone resorption. 20th annual lorne protein conference.
·
k. nash, m. feldmuller, j. de jersey, p. alewood and s.
hamilton (1994) kinetic characterization of a purple acid phosphatase: an
enzyme involved in bone resorption.19th annual lorne protein conference,
1994.
·
k. nash, m. feldmuller, j. de jersey, p. alewood and s.
hamilton (1993) continuous and discontinuous assays for phosphotyrosyl
protein phosphatases using phosphotyrosyl peptide substrates.37th annual
conference of the australian society for biochemistry and molecular biology
Acceuil
suivante
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